Glucocorticoid metabolism in the bovine cumulus–oocyte complex matured in vitro
نویسندگان
چکیده
منابع مشابه
Effect of Demecolicne Treatment on The Developmental Competency of In Vitro Matured Bovine Oocytes
Purpose: The aim of this study was to investigate whether demecolicne treatment of matured bovine oocytes adversely affects the process of in vitro fertilization and embryo development. Materials and Methods: Bovine Cumulus Oocyte Complexes (COC's) were matured in vitro and then were randomly allocated to two treatment groups of common concentrations of demecolicne (0.05 and 0.4 mg/ml for 30 mi...
متن کاملCumulus cell expansion and ultrastructural changes in in vitro matured bovine oocytes under heat stress
Cumulus-oocyte complexes (COCs) from cows were matured under normal (38.5°C) and elevated temperatures (41°C) simulating heat stress and their maturation was assessed based on measurement of cumulus expansion in both groups. There was a significant reduction (P
متن کاملEffect of Conjugated Linoleic Acid On Gene Expressions Involved in Lipid Metabolism in In Vitro Matured Sheep Oocytes
The aim of this study was to investigate the effect of different concentrations of conjugated linoleic acid (CLA) on in vitro maturation (IVM) and lipid metabolism-related genes in oocytes. In vitro maturation of oocyte was performed in the presence of control (0.0), 50, 100, and 200 µM CLA. Nuclear status and mRNA abundance of selected genes were evaluated following 24 h of IVM. Following the ...
متن کاملIn vitro fertilization and development of in vitro matured bovine follicular oocytes.
Bovine follicular oocytes matured in vitro were fertilized in vitro using epididymal spermatozoa from five different bulls and then cultured to the blastocyst stage in vitro. The fertilization rate, based on one pair of pronuclei and presence of one sperm tail, ranged from 55.2 to 64.3%. Embryo development (cleavage to blastocyst stage) ranged from 21.4 to 31.0% of the cultured ova reaching 8 c...
متن کاملStimulation of development of in vitro-matured and in vitro-fertilized bovine embryos by platelets.
In vitro-fertilized bovine embryos were incubated in Menezo's B2 medium (MB2) supplemented with 2 mg/mL of BSA. In Exp. 1, eight-cell stage embryos were allotted to one of the following groups: control medium (MB2), MB2 with 20 ng/mL of platelet-activating factor (PAF), 1 x 10(7) bovine blood platelets (Platelets), oviductal cells (BOEC), BOEC and 20 ng/mL of PAF (BOEC+PAF), or BOEC and 1 x 10(...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: REPRODUCTION
سال: 2016
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep-15-0363